Bioconversion of red ginseng saponines in the gastro-intestinal tract in-vitro model studied by high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry

A high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry (HPLC-FTICR-MS) method was developed to investigate the metabolism of ginsenosides in in vitro models of the gastro-intestinal tract. The metabolites were identified by high-resolution tandem mass spectrometry. Degradation and bioconversion routes of the different ginsenosides at acidic (gastric) conditions and in the presence of intestinal microbiota were elaborated. Besides hydrolysis (deglycosylation) also hydration reactions occurred at acidic conditions. The results illustrate the value of metabolite profiling by HPLC-FTICR-MS for understanding of the mechanisms in bioavailability of herbal drugs and their metabolites.

 

Authors: 
H. Kong, M. Wang, K. Venema, A. Maathuis, R. van der Heijden, J. van der Greef, G. Xu, T. Hankemeier
Authors from the NMC: 
Publication data (text): 
2009
DOI: 
10.1016/j.chroma.2008.11.030
Pages: 
2009; 1216 (11): 2195-2203
Published in: 
Journal of Chromatography A
Date of publication: 
March, 2009
Status of the publication: 
Published/accepted
Source: 
Sino Dutch Institute for Preventive and Personalized Medicine